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Mutations in PRPF31 are responsible for autosomal dominant retinitis pigmentosa (adRP, RP11 form) and affected families show nonpenetrance. Differential expression of the wildtype PRPF31 allele is responsible for this phenomenon: coinheritance of a mutation and a higher expressing wildtype allele provide protection against development of disease. It has been suggested that a major modulating factor lies in close proximity to the wildtype PRPF31 gene on Chromosome 19, implying that a cis-acting factor directly alters PRPF31 expression. Variable expression of CNOT3 is one determinant of PRPF31 expression. This study explored the relationship between CNOT3 (a trans-acting factor) and its paradoxical cis-acting nature in relation to RP11. Linkage analysis on Chromosome 19 was performed in mutation-carrying families, and the inheritance of the wildtype PRPF31 allele in symptomatic-asymptomatic sibships was assessed-confirming that differential inheritance of wildtype chromosome 19q13 determines the clinical phenotype (P < 2.6 × 10(-7) ). A theoretical model was constructed that explains the apparent conflict between the linkage data and the recent demonstration that a trans-acting factor (CNOT3) is a major nonpenetrance factor: we propose that this apparently cis-acting effect arises due to the intimate linkage of CNOT3 and PRPF31 on Chromosome 19q13-a novel mechanism that we have termed "linked trans-acting epistasis."

Original publication

DOI

10.1111/ahg.12042

Type

Journal article

Journal

Ann Hum Genet

Publication Date

01/2014

Volume

78

Pages

62 - 71

Keywords

CNOT3, PRPF31, epistasis, retinitis pigmentosa, Alleles, Chromosomes, Human, Pair 19, Computational Biology, Epistasis, Genetic, Eye Proteins, Female, Genes, Dominant, Genes, Recessive, Genetic Linkage, Genetic Loci, Heterozygote, Humans, Male, Microsatellite Repeats, Mutation, Pedigree, Pneumonia, Aspiration, Polymorphism, Genetic, Retinitis Pigmentosa, Transcription Factors