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Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterization of phagosomes from RAW 264.7 and bone marrow derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicate that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1. Moreover, bone marrow derived macrophages phagosomes mature considerably faster by fusion with endosomes and the lysosome which we validated using fluorogenic phagocytic assays. We provide a valuable resource for researcher in the field and recommend careful use of the RAW 264.7 cell line when studying phagosome functions. All MS data have been deposited in the ProteomeXchange with identifier PXD001293 (http://proteomecentral.proteomexchange.org/dataset/PXD001293).

Original publication

DOI

10.1002/pmic.201400431

Type

Journal article

Journal

Proteomics

Publication Date

09/2015

Volume

15

Pages

3169 - 3174

Keywords

Cell biology, Macrophage, Phagosome, Proteomics RAW 264.7, Animals, Cells, Cultured, Databases, Protein, Macrophages, Mice, Phagosomes, Protein Interaction Maps, Proteome, Proteomics, RAW 264.7 Cells