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Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.

Original publication

DOI

10.1371/journal.pone.0184496

Type

Journal article

Journal

PLoS One

Publication Date

2017

Volume

12

Keywords

Antiviral Agents, CD8-Positive T-Lymphocytes, Endocytosis, HIV Infections, Humans, Lymphocyte Depletion, Protein Multimerization, Ribosome Inactivating Proteins, Type 1, Saporins